Proteinase, Bacillus subtilis, sutilains
Proteinase, Bacillussubtilis, sutilains (9CI)
CAS: 12211-28-8
Molecular Formula: Proteinase, Bacillussubtilis, sutilains (9CI)
Proteinase, Bacillus subtilis, sutilains - Names and Identifiers
Proteinase, Bacillus subtilis, sutilains - Physico-chemical Properties
| Molecular Formula | Proteinase, Bacillussubtilis, sutilains (9CI) |
| Molar Mass | 420.458 |
Proteinase, Bacillus subtilis, sutilains - Application of Bacillus subtilis enzyme in industrial production
from VIP
Author:
Abstract:
Bacillus subtilis (Bacillus subtilis) is one of the main industrial enzyme production strains, because of its high enzyme production, variety, safety, environmental protection and other advantages are widely used in modern industrial production, the fermentation production of enzymes in food, feed, washing, textile, leather, paper, medicine and other fields are playing a very important role. In this paper, the application of Bacillus subtilis industrial enzyme was reviewed in detail, and its development trend was prospected.
Key words:
Bacillus subtilis enzyme industrial production Application Outlook
DOI:
10.3969/j.issn.1002-4026.2006.03.014
cited:
year:
2006
Last Update：2024-01-02 23:10:35
Proteinase, Bacillus subtilis, sutilains - Reduction performance of Bacillus subtilis enzyme and its application in indigo dye reduction
from VIP Journal Professional Edition
Author:
Liao Huan , Wu Yanli , Zhou Ting , Chen Jianfang
Abstract:
Bacillus subtilis was cultured with yeast extract powder, peptone, agarose powder and other materials under specific conditions to secrete Bacillus subtilis enzymes, including extracellular enzymes and intracellular enzymes; the electrical conductivity and reducing ability of the enzyme were tested. The results showed that the intracellular enzyme of Bacillus subtilis had reducing ability. When the applied voltage of the power supply was adjusted to 15V, the reduction potential of the intracellular enzyme was 867, more than the required reduction potential of indigo -760, Bacillus subtilis intracellular enzyme can be used as a reducing agent for Indigo electrochemical staining system, which is used for Indigo electrochemical reduction staining, it was found that when the enzyme system temperature was 60 ℃,pH was 11, and the reduction time was 40 min, the reduction effect of indigo dye was better.
Key words:
electrochemical staining Bacillus subtilis enzyme indigo dye preparation
DOI:
CNKI:SUN:GCHZ.0.2020-01-015
year:
2020
Last Update：2023-08-16 22:05:47
Proteinase, Bacillus subtilis, sutilains - Application of Bacillus subtilis enzyme in industrial production
from VIP Journal Professional Edition
Author:
Abstract:
Bacillus subtilis (Bacillus subtilis) is one of the main industrial enzyme production strains, because of its high enzyme production, variety, safety, environmental protection and other advantages are widely used in modern industrial production, the fermentation production of enzymes in food, feed, washing, textile, leather, paper, medicine and other fields are playing a very important role. In this paper, the application of Bacillus subtilis industrial enzyme was reviewed in detail, and its development trend was prospected.
Key words:
Bacillus subtilis enzyme industrial production Application Outlook
DOI:
CNKI:SUN:SDKX.0.2006-03-006
year:
2006
Last Update：2023-08-16 22:05:47
Proteinase, Bacillus subtilis, sutilains - Direct bilirubin assay kit with Bacillus subtilis laccase
from Palm Bridge research
Application (patent) number:
CN202010140961.8
application date:
2020-03-03
Public/Announcement Number:
CN111455018A
Public/announcement date:
2020.07.28
applicant (patent):
Tianjin University
inventor:
correct , You Shengping , Zhang Chengyu , Su Rongxin
National and provincial code:
CN120112
Abstract:
The invention discloses a direct bilirubin detection kit containing Bacillus subtilis laccase, which is composed of reagent R1, reagent R2, reagent R3 and reagent R4: buffer solution 10250mmol/L, mannitol 0.22 mmol/L, sodium nitrite 0.050.2g/L, sodium azide 0.0015g/L, Sodium edetate g/L, the rest is water; Reagent R2 includes: buffer 600 mmol/L,KCl mmol/L, polyethylene glycol 5500G/L, balance water; Reagent R3: Bacillus subtilis laccase; Reagent R4: direct bilirubin standard. The fermentation cycle of Bacillus subtilis laccase in the kit is short, the production cost is low, and the rapid mass production can be realized. The kit has good stability and low cost for detecting samples.
Last Update：2023-08-16 22:05:47
Proteinase, Bacillus subtilis, sutilains - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Low temperature applicability of enzyme | Japan and other parts of Asia are used to washing at room temperature, therefore, room temperature washing has been a new topic in recent years for subtilisin (Bacillus subtilis enzyme). The properties of some psychrophilic enzymes produced from Antarctic bacteria have been known using protein modeling and X-ray crystallography methods. Studies have shown that the intramolecular interaction of the residues of the psychrophilic enzyme is weakened, and in some cases the interaction with the solvent is enhanced, resulting in a more flexible molecular structure with less energy loss in catalysis. |
| enzyme specificity | by changing the residues of subtilisin (Bacillus subtilis enzyme), the specificity of the enzyme may be influenced. The residues Glul56 and Gly166 located in the binding pocket of subtilisin (Bacillus subtilis) S1 alter the side chain volume and hydrophobicity of Gly166 by means of site-directed mutagenesis, or binding to lGul56 confers different charge properties on the substrate specificity of the enzyme. |
| enzyme activity | the magnitude of the catalytic capacity of the enzyme is a key indicator of the enzyme, the catalytic efficiency of the enzyme is generally expressed in Kcat/Km. The substitution of Lle31 in subtilisin (Bacillus subtilis enzyme) E to val increased Kcat several fold. Subtilisin (Bacillus subtilis enzyme) BL, in which vall04 was replaced with intestinal r, greatly increased the activity of the four peptides and increased the hydrolysis of casein by 26%. |
| production method | was obtained by fermentation of a strain of the genus bacillus (No. BS-208) selected from a soil sample. |
Last Update：2024-04-09 02:00:04
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